Stem cell research

Stem Cell Research focused around hyperbaric oxygen dives done at the 2.0 ATA* and 2.5 ATA**.
CD34+/CD45-dim stem cell mobilization by hyperbaric oxygen — Changes with oxygen dosag

* 2 ATA equals 33 feet below the surface. (A 800% increase in stem cells at 2 ATA)
** 2.5 ATA equals 50 feet below the surface. (A 2,400% increase in stem cells at 2.5 ATA)

Abstract  Because hyperbaric oxygen treatment mobilizes bone marrow derived-stem/progenitor cells by a free radical mediated mechanism, we hypothesized that there may be differences in mobilization efficiency based on exposure to different oxygen partial pressures. Blood from twenty consecutive patients was obtained before and after the 1st, 10th and 20th treatment at two clinical centers using protocols involving exposures to oxygen at either 2.0 or 2.5 atmospheres absolute (ATA). Post-treatment values of CD34+, CD45-dim leukocytes were always 2-fold greater than the pre-treatment values for both protocols. Values for those treated at 2.5 ATA were significantly greater than those treated at 2.0 ATA by factors of 1.9 to 3-fold after the 10th and before and after the 20th treatments. Intracellular content of hypoxia inducible factors −1, −2, and −3, thioredoxin-1 and poly-ADP-ribose polymerase assessed in permeabilized CD34+ cells with fluorophore-conjugated antibodies were twice as high in all post- versus pre-treatment samples with no significant differences between 2.0 and 2.5 ATA protocols. We conclude that putative progenitor cell mobilization is higher with 2.5 versus 2.0 ATA treatments, and all newly mobilized cells exhibit higher concentrations of an array of regulatory proteins.

© 2014 The Authors. Published by Elsevier B.V

Stem cell research
Stem Cell Research with hyperbaric oxygen dives done at 2.0 ATA and 2.5 ATA.
Chemical detox
A photo of heavy metals deposited on an oxygen hood neck dam. This was after two hours at 2.5 ATA